- Configure Your
- Bioconjugation
- Program
Xcellon delivers site-specific, homogeneous bioconjugates—from ADCs to antibody-oligonucleotide conjugates—with precision chemistry and speed-to-clinic focus.
- XSPEC™ — Step-By-Step Configuration
Build Your Bioconjugation Program
Configure up to 9 conjugates side-by-side, then submit a unified inquiry to our team.
Stochastic Cysteine Conjugation
Cysteine conjugation exploits the unique nucleophilicity of the thiol (–SH) side chain on cysteine residues. Under mild, controlled conditions, free thiols react selectively with thiol-reactive electrophiles—most commonly maleimides, haloacetamides, and vinyl sulfones—forming stable thioether or thiosuccinimide bonds.
In native IgG antibodies, the four interchain disulfide bonds can be selectively reduced using TCEP or DTT to liberate free cysteines at defined positions, enabling conjugation with a theoretical DAR of up to 8. Partial reduction protocols yield mixed DAR distributions; careful stoichiometry control can produce a mixture resulting in a lower-DAR product.
Cysteine conjugation is compatible with a broad range of cytotoxic payloads and is validated across multiple approved ADC platforms.
Glycan Conjugation
Fc glycan remodeling enables site-specific conjugation through the conserved N-linked glycan at Asn297 of IgG antibodies.
Chemoenzymatic approaches using endoglycosidases and engineered glycosynthases can install bioorthogonal handles such as azido sugars for subsequent click-based payload attachment. Compared with traditional lysine conjugation, glycan-mediated approaches typically produce narrower DAR distributions and improved conjugate homogeneity while avoiding direct modification of the antigen-binding region.
In addition, enzymatic remodeling introduces added process and analytical complexity relative to more established cysteine-based site-specific conjugation methods. Despite these challenges, Fc glycan conjugation remains an increasingly attractive platform for highly defined ADCs and next-generation antibody conjugates including AOCs, ISACs, and radioconjugates. Xcellon Biologics has developed the needed process methods to deliver these conjugates on the scales needed to support our clients' programs.
Enzymatic Site-Specific Conjugation via Microbial Transglutaminase
Microbial transglutaminase (mTGase) catalyzes acyl-transfer reactions between glutamine residues and primary amine-containing substrates, forming stable isopeptide linkages under mild conditions. In ADC development, engineered glutamine motifs such as LLQGA or accessible Fc glutamine residues including Q295 can serve as site-selective conjugation handles.
Because Q295 is partially shielded by the conserved Fc glycan, many workflows require prior deglycosylation to enable efficient conjugation. Under optimized conditions, mTGase-mediated conjugation can generate highly homogeneous ADCs with controlled DARs, most commonly DAR 2 or DAR 4. Xcellon Biologics has developed the needed process methods to deliver these conjugates on the scales needed to support our clients' programs.
Engineered Cysteine Mutants for Precision ADC Synthesis
ThioMab and related engineered cysteine platforms enable site-specific ADC conjugation through the introduction of strategically positioned cysteine residues within the antibody scaffold. These engineered thiols provide defined conjugation handles that reduce the heterogeneity associated with conventional stochastic interchain cysteine conjugation methods.
Emerging & Custom Bioconjugation Approaches
Beyond our core platform chemistries, Xcellon supports a growing portfolio of emerging and bespoke bioconjugation strategies tailored to unique program requirements.
Bio-orthogonal Click Chemistry: Incorporation of non-canonical amino acids via amber suppression enables CuAAC or strain-promoted copper-free click conjugation. Tetrazine-TCO ligation offers some of the fastest bio-orthogonal reaction rates known.
SpyCatcher / SpyTag: Isopeptide bond-forming protein tag systems allow modular, covalent assembly of antibody fragments with payloads or effector domains—useful for bispecific and multimerization strategies.
NHS Ester (Lysine) Conjugation: For programs where DAR heterogeneity is acceptable and rapid turnaround is prioritized, conventional NHS ester conjugation remains cost-effective with a well-understood regulatory history.
Sortase A & Butelase: Transpeptidase-mediated ligation at C- or N-terminal recognition sequences enables site-specific attachment of peptide-payload cassettes with full sequence control.
start configuring your program.
All Conjugates Configured
Review your program summary and submit a unified inquiry to the Xcellon team.
TERMS AND CONDITIONS
These Standard Terms and Conditions, together with the applicable Statement of Work (“SOW”), constitute the entire agreement between Xcellon Biologics LLC (“Xcellon”) and you, the Customer, with respect to the Services described therein and supersede all prior discussions or agreements relating to the Project. The exception to this is the Confidentiality Agreement between both Parties signed.
1. Services
Xcellon shall perform the services described in the SOW using commercially reasonable efforts and in accordance with applicable law and industry standards. Xcellon shall notify Customer if it determines that performance of the Services is likely to be substantially delayed or is not scientifically or technically feasible. Xcellon may use its Affiliates as subcontractors in the performance of the Services and shall remain responsible for their performance.
Any changes to the scope, cost, or timing of the Services shall be agreed in writing by both parties prior to implementation. Xcellon shall maintain appropriate records documenting the work performed and results achieved in a manner consistent with good scientific practice.
2. Fees and Payment
Customer shall pay Xcellon the fees set forth in the SOW. Xcellon shall invoice in accordance with the payment schedule specified therein. All undisputed invoices shall be payable within thirty (30) days of receipt (“Due Date”). Late payments may accrue interest at the rate of one and one half percent (1.5%) per month or the maximum amount permitted by law, whichever is less, on the outstanding balance from the Due Date until paid in full. Customer shall be responsible for all sales, use, value added, or similar taxes applicable to the Services, other than taxes based on Xcellon’s income. If any invoice remains unpaid for more than fifteen (15) days after the Due Date, Xcellon may, in addition to any other rights or remedies: (i) suspend performance of Services under the applicable SOW until all outstanding amounts are paid in full, and/or (ii) terminate the SOW in accordance with Section 3. Any suspension of Services due to Customer’s non-payment shall not relieve Customer of its payment obligations for Services already performed or costs already incurred, and shall extend all project timelines and delivery dates by the duration of the suspension.
3. Term and Termination
Either party may terminate the SOW for material breach upon thirty (30) days written notice and opportunity to cure. Customer may terminate the SOW for convenience upon thirty (30) days written notice. Xcellon may terminate the SOW upon written notice if it reasonably determines that completion of the Project is not scientifically or technically feasible. Upon termination, Customer shall pay Xcellon for all reasonable Services performed and costs incurred through the effective date of termination.
4. Transfer of Customer Materials
Customer agrees to provide to Xcellon on a non-exclusive basis Customer Materials and Customer Information. For purposes of this Agreement, the term “Customer Materials” shall mean the materials provided by Customer and any and all progeny, analogs, derivatives, complexes, compositions, or subunits thereof, and all associated information and technology related thereto and “Customer Information” shall mean any information provided to Xcellon in pursuit of services from Xcellon that are entered into the Xcellon website or provided in oral or written form to Xcellon in the course of requesting or executing services from Xcellon. Customer retains all right, title, and interest in such materials. Xcellon shall use Customer Materials and Customer Information solely for the purpose of performing the Services and shall handle, store, and dispose of such materials using reasonable care and in accordance with applicable law. All Customer Materials are provided “as is” without any warranties, express or implied. Upon Customer’s request, unused materials shall be returned or disposed of at Customer’s election.
5. Confidentiality
Each party agrees to maintain the confidentiality of all non-public information disclosed by the other party that is identified as confidential or that reasonably should be understood to be confidential. The receiving party shall use such Confidential Information solely for purposes of performing its obligations under the SOW and shall not disclose such information to any third party except to its employees or contractors who have a need to know and are bound by confidentiality obligations at least as protective as those set forth herein.
Confidential Information does not include information that is publicly available through no fault of the receiving party, independently developed without use of the Confidential Information, or lawfully obtained from a third party without restriction. Confidentiality obligations shall survive for five (5) years following termination of the SOW, except with respect to trade secrets, which shall remain protected for so long as they remain trade secrets.
6. Intellectual Property
a) All inventions (whether patentable or not), patents, know-how, trademarks, information, data, writings and other property in any form whatsoever, or improvements upon, which were owned or controlled by Xcellon (“Xcellon Background Intellectual Property”) or Customer (“Customer Background Intellectual Property”) prior to the Effective Date of this Agreement, or were or are subsequently developed independent from this Agreement, shall remain the property of such party. Except for Xcellon Background Intellectual Property, Xcellon hereby irrevocably assigns to Customer all right, title, and interest in inventions, discoveries, data, and deliverables generated in the performance of the Services (“Customer Inventions”). For clarity, any inventions, improvements, works of authorship, mask works, ideas or information derived from Customer’s Background Intellectual Property as defined in Exhibit A belongs to Customer and not Xcellon.
To the extent Xcellon incorporates any Xcellon Background Intellectual Property into Customer Inventions, Xcellon grants Customer a nonexclusive, royalty free, perpetual, irrevocable, transferable, worldwide license, with the right to sublicense, to use such intellectual property solely in connection with exploitation of the Customer Inventions.
Notwithstanding the foregoing, Xcellon may use anonymized and aggregated data derived from the performance of the Services solely for internal statistical analysis and process improvement purposes, provided such use does not disclose Customer Confidential Information or enable identification of Customer, its materials, or the Service Inventions.
7. Representations and Warranties
Each party represents and warrants that it has the full power and authority to enter into and perform this agreement and that its performance does not violate any agreement or obligation to any third party. Xcellon represents that it has all necessary licenses and authorizations required to perform the Services. Xcellon makes no warranty regarding the success of any scientific research or achievement of any particular result.
8. Indemnification
Xcellon shall indemnify, defend, and hold harmless Customer from third party claims arising out of (i) Xcellon’s material breach of this agreement or (ii) the gross negligence or willful misconduct of Xcellon. Customer shall indemnify, defend, and hold harmless Xcellon from third party claims arising out of (i) Customer’s use of the deliverables, (ii) Customer’s materials, or (iii) Customer’s material breach of this agreement. The indemnified party shall promptly notify the indemnifying party of any claim and permit the indemnifying party to control the defense and settlement of such claim, provided that no settlement admitting liability on behalf of the indemnified party shall be made without the indemnified party’s prior written consent.
Notwithstanding anything to the contrary herein, the maximum aggregate liability of Xcellon under this Section 8 shall not exceed the total fees paid or payable to Xcellon under the applicable SOW. This limitation shall not apply to claims arising from Xcellon’s willful misconduct or breach of confidentiality obligations.
9. Disclaimer and Limitation of Liability
Except as expressly set forth herein, neither party makes any warranties, express or implied, including any warranties of merchantability or fitness for a particular purpose. Neither party shall be liable for any indirect, incidental, special, or consequential damages. Except for indemnification obligations or breach of confidentiality, the maximum aggregate liability of either party shall not exceed the fees paid to Xcellon under the applicable SOW.
10. Independent Contractor
The parties are independent contractors and nothing herein shall be deemed to create any partnership, joint venture, or agency relationship.
11. Governing Law
This agreement shall be governed by and construed in accordance with the laws of the State of Delaware, without regard to its conflict of laws principles.
12. Force Majeure
Neither party shall be liable for delays or failure to perform (except payment obligations) caused by circumstances beyond its reasonable control, including natural disasters, pandemics, war, government actions, or supply failures. The affected party shall promptly notify the other party and use reasonable efforts to resume performance. Either party may terminate if the event prevents performance for more than sixty (60) days, with Customer paying for all Services performed through termination.
13. Entire Agreement
The SOW and these Standard Terms constitute the entire agreement between the parties with respect to the subject matter hereof and supersede all prior agreements or understandings relating thereto.
Terms and Conditions 5.28.2026
Tell Us Exactly What You Need
Skip the step-by-step configurator and describe your program in your own words. Our scientific team will review your specifications and follow up with a tailored proposal within one business day.
TERMS AND CONDITIONS
These Standard Terms and Conditions, together with the applicable Statement of Work (“SOW”), constitute the entire agreement between Xcellon Biologics LLC (“Xcellon”) and you, the Customer, with respect to the Services described therein and supersede all prior discussions or agreements relating to the Project. The exception to this is the Confidentiality Agreement between both Parties signed.
1. Services
Xcellon shall perform the services described in the SOW using commercially reasonable efforts and in accordance with applicable law and industry standards. Xcellon shall notify Customer if it determines that performance of the Services is likely to be substantially delayed or is not scientifically or technically feasible. Xcellon may use its Affiliates as subcontractors in the performance of the Services and shall remain responsible for their performance.
Any changes to the scope, cost, or timing of the Services shall be agreed in writing by both parties prior to implementation. Xcellon shall maintain appropriate records documenting the work performed and results achieved in a manner consistent with good scientific practice.
2. Fees and Payment
Customer shall pay Xcellon the fees set forth in the SOW. Xcellon shall invoice in accordance with the payment schedule specified therein. All undisputed invoices shall be payable within thirty (30) days of receipt (“Due Date”). Late payments may accrue interest at the rate of one and one half percent (1.5%) per month or the maximum amount permitted by law, whichever is less, on the outstanding balance from the Due Date until paid in full. Customer shall be responsible for all sales, use, value added, or similar taxes applicable to the Services, other than taxes based on Xcellon’s income. If any invoice remains unpaid for more than fifteen (15) days after the Due Date, Xcellon may, in addition to any other rights or remedies: (i) suspend performance of Services under the applicable SOW until all outstanding amounts are paid in full, and/or (ii) terminate the SOW in accordance with Section 3. Any suspension of Services due to Customer’s non-payment shall not relieve Customer of its payment obligations for Services already performed or costs already incurred, and shall extend all project timelines and delivery dates by the duration of the suspension.
3. Term and Termination
Either party may terminate the SOW for material breach upon thirty (30) days written notice and opportunity to cure. Customer may terminate the SOW for convenience upon thirty (30) days written notice. Xcellon may terminate the SOW upon written notice if it reasonably determines that completion of the Project is not scientifically or technically feasible. Upon termination, Customer shall pay Xcellon for all reasonable Services performed and costs incurred through the effective date of termination.
4. Transfer of Customer Materials
Customer agrees to provide to Xcellon on a non-exclusive basis Customer Materials and Customer Information. For purposes of this Agreement, the term “Customer Materials” shall mean the materials provided by Customer and any and all progeny, analogs, derivatives, complexes, compositions, or subunits thereof, and all associated information and technology related thereto and “Customer Information” shall mean any information provided to Xcellon in pursuit of services from Xcellon that are entered into the Xcellon website or provided in oral or written form to Xcellon in the course of requesting or executing services from Xcellon. Customer retains all right, title, and interest in such materials. Xcellon shall use Customer Materials and Customer Information solely for the purpose of performing the Services and shall handle, store, and dispose of such materials using reasonable care and in accordance with applicable law. All Customer Materials are provided “as is” without any warranties, express or implied. Upon Customer’s request, unused materials shall be returned or disposed of at Customer’s election.
5. Confidentiality
Each party agrees to maintain the confidentiality of all non-public information disclosed by the other party that is identified as confidential or that reasonably should be understood to be confidential. The receiving party shall use such Confidential Information solely for purposes of performing its obligations under the SOW and shall not disclose such information to any third party except to its employees or contractors who have a need to know and are bound by confidentiality obligations at least as protective as those set forth herein.
Confidential Information does not include information that is publicly available through no fault of the receiving party, independently developed without use of the Confidential Information, or lawfully obtained from a third party without restriction. Confidentiality obligations shall survive for five (5) years following termination of the SOW, except with respect to trade secrets, which shall remain protected for so long as they remain trade secrets.
6. Intellectual Property
a) All inventions (whether patentable or not), patents, know-how, trademarks, information, data, writings and other property in any form whatsoever, or improvements upon, which were owned or controlled by Xcellon (“Xcellon Background Intellectual Property”) or Customer (“Customer Background Intellectual Property”) prior to the Effective Date of this Agreement, or were or are subsequently developed independent from this Agreement, shall remain the property of such party. Except for Xcellon Background Intellectual Property, Xcellon hereby irrevocably assigns to Customer all right, title, and interest in inventions, discoveries, data, and deliverables generated in the performance of the Services (“Customer Inventions”). For clarity, any inventions, improvements, works of authorship, mask works, ideas or information derived from Customer’s Background Intellectual Property as defined in Exhibit A belongs to Customer and not Xcellon.
To the extent Xcellon incorporates any Xcellon Background Intellectual Property into Customer Inventions, Xcellon grants Customer a nonexclusive, royalty free, perpetual, irrevocable, transferable, worldwide license, with the right to sublicense, to use such intellectual property solely in connection with exploitation of the Customer Inventions.
Notwithstanding the foregoing, Xcellon may use anonymized and aggregated data derived from the performance of the Services solely for internal statistical analysis and process improvement purposes, provided such use does not disclose Customer Confidential Information or enable identification of Customer, its materials, or the Service Inventions.
7. Representations and Warranties
Each party represents and warrants that it has the full power and authority to enter into and perform this agreement and that its performance does not violate any agreement or obligation to any third party. Xcellon represents that it has all necessary licenses and authorizations required to perform the Services. Xcellon makes no warranty regarding the success of any scientific research or achievement of any particular result.
8. Indemnification
Xcellon shall indemnify, defend, and hold harmless Customer from third party claims arising out of (i) Xcellon’s material breach of this agreement or (ii) the gross negligence or willful misconduct of Xcellon. Customer shall indemnify, defend, and hold harmless Xcellon from third party claims arising out of (i) Customer’s use of the deliverables, (ii) Customer’s materials, or (iii) Customer’s material breach of this agreement. The indemnified party shall promptly notify the indemnifying party of any claim and permit the indemnifying party to control the defense and settlement of such claim, provided that no settlement admitting liability on behalf of the indemnified party shall be made without the indemnified party’s prior written consent.
Notwithstanding anything to the contrary herein, the maximum aggregate liability of Xcellon under this Section 8 shall not exceed the total fees paid or payable to Xcellon under the applicable SOW. This limitation shall not apply to claims arising from Xcellon’s willful misconduct or breach of confidentiality obligations.
9. Disclaimer and Limitation of Liability
Except as expressly set forth herein, neither party makes any warranties, express or implied, including any warranties of merchantability or fitness for a particular purpose. Neither party shall be liable for any indirect, incidental, special, or consequential damages. Except for indemnification obligations or breach of confidentiality, the maximum aggregate liability of either party shall not exceed the fees paid to Xcellon under the applicable SOW.
10. Independent Contractor
The parties are independent contractors and nothing herein shall be deemed to create any partnership, joint venture, or agency relationship.
11. Governing Law
This agreement shall be governed by and construed in accordance with the laws of the State of Delaware, without regard to its conflict of laws principles.
12. Force Majeure
Neither party shall be liable for delays or failure to perform (except payment obligations) caused by circumstances beyond its reasonable control, including natural disasters, pandemics, war, government actions, or supply failures. The affected party shall promptly notify the other party and use reasonable efforts to resume performance. Either party may terminate if the event prevents performance for more than sixty (60) days, with Customer paying for all Services performed through termination.
13. Entire Agreement
The SOW and these Standard Terms constitute the entire agreement between the parties with respect to the subject matter hereof and supersede all prior agreements or understandings relating thereto.
Terms and Conditions 5.28.2026
- Our Process
From Configuration To Conjugate
1.
Submit your program configuration and receive a tailored proposal within 1 business day.
2.
Feasibility review with your dedicated Xcellon scientist — alignment on chemistry, timeline, and deliverables.
3.
Conjugation execution with progress updates and intermediate analytical data.
4.
Full analytical package delivery: CoA, spectral data, DAR distribution, and SEC/HIC chromatograms.
5.
Cryogenic shipment of final conjugate with QC release documentation — ready for your downstream assays.